Abstract: The temperature is important parameter in the greenhouses, precise, stable temperature can ensure the normal growth of vegetables, this paper proposes a greenhouse temperature control system based on the CAN bus for the traditional RS-485 bus defects.The single-chip microcomputer is used as the core to established greenhouse temperature monitoring system of the node and network structure with CAN bus technology and temperature sensor DS18B20, and gives the overall structure of the system and the key software process.The research indicates that the control system has good versatility, high temperature control precision, strong anti-interference ability, it can satisfy the real time measurement and control requirements.