Abstract: In order to mitigate the lower accuracy of node localization in wireless sensor network,a new algorithm NLGS (Node Localization based on Glowworm Swarm) is proposed by glowworm swarm algorithm.In this algorithm,the calculation method of unknown node localization in three-dimensional space and the error optimization model is presented with anchor at first,and the objective function is solved by glowworm swarm algorithm,which is to achieve the fast convergence,a simulation with OPNET was conducted to study the key influence factors of NLGS.The result shows that,compared to other algorithm,NLGS has better adaptability.