Abstract: Since Antifuse-FPGA is only used in specified fields and it's difficult to manufacture,researches about bit-stream generation of Antifuse -FPGA are seldom done before.Basic knowledge about Antifuse-FPGA and the flow of FPGA CAD tool were introduced firstly.Then detail architecture of Antifuse-FPGA was described and methods of configuring Antifuse - FPGA were also elaborated through a real example.Afterward this paper discussed the way to configure Antifuse-FPGA,and by doing that the pattern of bit-stream file and its basic unit, containing information of Antifuse which was to be programmed,was deduced.At last,the algorithm of generating bit - stream file of Antifuse-FPGA was concluded and it was realized on the experimental platform.