Abstract: With the rapid development of wireless sensor networks and technologies, the need for high-precision wireless time-frequency synchronization technology in the collaborative work of distributed systems is increasingly urgent. In response to the demand for high-precision wireless time-frequency synchronization in the line-of-sight distributed network under the condition of satellite rejection, this paper proposes a clock synchronization system scheme based on carrier bidirectional frequency transfer. It innovatively implements the mutual transmission of time-frequency information through millimeter-wave channels by deploying a full-duplex bidirectional time-frequency synchronization protocol, and introduces Xilinx MMCM IP dynamic phase shift function core to control the phase of time-frequency signals and realize frequency difference measurement and dynamic phase shift. This effectively improves the phase shift performance of the time-frequency synchronization architecture, and finally realizes a complete set of sub-nanosecond-level ultra-high-precision wireless time-frequency synchronization scheme. The technical architecture, RF front-end, wireless channel transmission and anti-jamming capability of the entire system are modeled and simulated in this paper, which verifies the effectiveness of the entire technical solution and the optimal phase shift accuracy. Besides, the 60 GHz RF front-end and Xilinx 7 series FPGA are also used to complete the principle prototype design. The experimental results have proved that the time-frequency synchronization system can provide wireless time-frequency mutual calibration service with a synchronization accuracy of up to 322.2 ps between nodes to achieve frequency synchronization and phase alignment, which can also support the development of various distributed collaborative work. Compared with traditional wireless synchronization methods, this scheme has higher precision, smaller influence from wireless channels and stronger anti-interference abilities, which is easy to extend to complex environments such as high dynamics and more suitable for wireless distributed networks.